Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 24
Page xix
... TEMPLATES BY BACTERIOPHAGE DNA - DEPENDENT RNA POLYMERASES 10.27 Plasmid Vectors for Preparing RNA Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific ...
... TEMPLATES BY BACTERIOPHAGE DNA - DEPENDENT RNA POLYMERASES 10.27 Plasmid Vectors for Preparing RNA Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific ...
Page 18-81
... template . However , many templates used to synthesize mRNAs in vitro are derived from cDNAs and carry tracts of poly ( dA : dT ) near their 3 ' termini . It is conceivable that the resulting tracts of poly ( A ) may confer stability on ...
... template . However , many templates used to synthesize mRNAs in vitro are derived from cDNAs and carry tracts of poly ( dA : dT ) near their 3 ' termini . It is conceivable that the resulting tracts of poly ( A ) may confer stability on ...
Page 1-13
... template ratios and nucleotide concentrations , effect on yield of radioactive probes , 10.20-10.21 repair of DNA to create blunt end , 8.27 , 17.20-17.23 synthesis of single - stranded DNA probes , 10.22-10.24 synthesis of uniformly ...
... template ratios and nucleotide concentrations , effect on yield of radioactive probes , 10.20-10.21 repair of DNA to create blunt end , 8.27 , 17.20-17.23 synthesis of single - stranded DNA probes , 10.22-10.24 synthesis of uniformly ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml