Molecular Cloning: A Laboratory Manual, Book 3 |
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Page F-2
... TERMINI Recessed 3 ' termini can be filled by the polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ( 1 ) the ...
... TERMINI Recessed 3 ' termini can be filled by the polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ( 1 ) the ...
Page F-1
... termini of the target fragment are identical to , or compatible with , those of the new vector . The target fragment can then be ligated to the vector without ... TERMINI Recessed 3 ' termini can Subcloning F.1 Appendix F: Subcloning.
... termini of the target fragment are identical to , or compatible with , those of the new vector . The target fragment can then be ligated to the vector without ... TERMINI Recessed 3 ' termini can Subcloning F.1 Appendix F: Subcloning.
Page F-2
... termini can be filled by the polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence ... termini at the end ( s ) of the DNA and ( 2 ) whether partial or complete filling is required . For example , to fill ...
... termini can be filled by the polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence ... termini at the end ( s ) of the DNA and ( 2 ) whether partial or complete filling is required . For example , to fill ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml