Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xxvi
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
Page 16-41
... transfection of viral genomes and plasmids carrying viral sequences . Although the mechanism of action of DEAE - dextran is not known , it is thought that the polymer might bind to DNA and inhibit the action of nucleases and / or bind ...
... transfection of viral genomes and plasmids carrying viral sequences . Although the mechanism of action of DEAE - dextran is not known , it is thought that the polymer might bind to DNA and inhibit the action of nucleases and / or bind ...
Page 16-42
... transfection was > 2 × 106 ( McCutchan and Pagano 1968 ) . Although this material is no longer available commercially , it is still occasionally found in chemical storerooms . The older batches of higher - molecular - weight DEAE ...
... transfection was > 2 × 106 ( McCutchan and Pagano 1968 ) . Although this material is no longer available commercially , it is still occasionally found in chemical storerooms . The older batches of higher - molecular - weight DEAE ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml