Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 52
Page 18-35
... tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 7. Recover the bottom microfuge tube , which should contain the cell ...
... tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 7. Recover the bottom microfuge tube , which should contain the cell ...
Page 18-38
... tube . This prevents the glass beads from exploding out of the tube during heating . 8. Heat the tube in a boiling - water bath for 3 minutes . 9. Transfer the glass beads into a fresh microfuge tube , and wash the original tube with ...
... tube . This prevents the glass beads from exploding out of the tube during heating . 8. Heat the tube in a boiling - water bath for 3 minutes . 9. Transfer the glass beads into a fresh microfuge tube , and wash the original tube with ...
Page 18-39
... tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain the cell ...
... tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain the cell ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml