Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-25
... usually by the calcium phosphate coprecipitation technique ( see pages 16.32-16.40 ) . If a selectable marker is not used , cells that have been successfully transfected can some- times be identified by their characteristic transformed ...
... usually by the calcium phosphate coprecipitation technique ( see pages 16.32-16.40 ) . If a selectable marker is not used , cells that have been successfully transfected can some- times be identified by their characteristic transformed ...
Page 18-37
... usually takes 20–30 minutes . 7. Collect the protoplasts by centrifugation at 500g for 10 minutes at 4 ° C . Discard the supernatant . 8. Resuspend the pellet ( which consists of protoplasts and intact cells ) in a volume of lysis ...
... usually takes 20–30 minutes . 7. Collect the protoplasts by centrifugation at 500g for 10 minutes at 4 ° C . Discard the supernatant . 8. Resuspend the pellet ( which consists of protoplasts and intact cells ) in a volume of lysis ...
Page 18-79
... usually optimal for translation of capped mRNAs . Approximately 40 mM KCl is usually optimal for translation of uncapped mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA synthesized in vitro can be ...
... usually optimal for translation of capped mRNAs . Approximately 40 mM KCl is usually optimal for translation of uncapped mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA synthesized in vitro can be ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml