Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-17
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
Page 16-23
... vectors have been developed that replicate episomally both in transformed mammalian cells and in bacteria ( DiMaio et al . 1982 ; Sarver et al . 1982 ; for review , see Campo 1985 ) . The basic properties of these vectors are : • They ...
... vectors have been developed that replicate episomally both in transformed mammalian cells and in bacteria ( DiMaio et al . 1982 ; Sarver et al . 1982 ; for review , see Campo 1985 ) . The basic properties of these vectors are : • They ...
Page 16-25
... vectors ( 8-14 kb ) and the dearth of unique cloning sites , the insertion of a foreign gene into BPV vectors is not always straightforward . Most vectors contain only one or two restriction sites at which the coding sequences of ...
... vectors ( 8-14 kb ) and the dearth of unique cloning sites , the insertion of a foreign gene into BPV vectors is not always straightforward . Most vectors contain only one or two restriction sites at which the coding sequences of ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml