Proteomics: From Protein Sequence to FunctionProteomics: From Protein Sequence to Function comprehensively reviews the exciting and emerging field of proteomics, the study of the protein products of the human genome. This book covers the current advances in genomics, describes existing methods for proteome analysis, and highlights the need for novel methods and instrumentation. It also presents the mechanisms of proteome data and the key applications of proteome analysis to areas such as drug discovery and cancer. |
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Page 12
... approach is to make protein products encoded by cDNA clones available for further analysis and use , such as the generation of protein arrays . This requires a highly parallel approach to protein expression analysis , including the ...
... approach is to make protein products encoded by cDNA clones available for further analysis and use , such as the generation of protein arrays . This requires a highly parallel approach to protein expression analysis , including the ...
Page 105
... approach can be applied to data- dependent LC - MS / MS analyses . In this approach , peptides separated by RP - HPLC are sprayed into an on - line mass spectrometer capable of data - dependent MS / MS ( i.e. the mass spectrometer ...
... approach can be applied to data- dependent LC - MS / MS analyses . In this approach , peptides separated by RP - HPLC are sprayed into an on - line mass spectrometer capable of data - dependent MS / MS ( i.e. the mass spectrometer ...
Page 176
... approach described above has been developed by scientists at Hoffmann - La Roche ( Langen et al . , 1998 ) . Instead of excising individual spots from a 2 - DE gel , entire gels are cut with a gridding tool into small pieces ( e.g. 1 ...
... approach described above has been developed by scientists at Hoffmann - La Roche ( Langen et al . , 1998 ) . Instead of excising individual spots from a 2 - DE gel , entire gels are cut with a gridding tool into small pieces ( e.g. 1 ...
Contents
Methods for measurement of gene mRNA expression | 23 |
Twodimensional polyacrylamide gel eletrophoresis for proteome analysis | 43 |
Detecting proteins in polyacrylamide gels and on electroblot membranes | 65 |
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2-DE gels Aebersold amino acid Anal analysis of protein analyzed antibodies antigen application approach automated Biochem Biol biological blotting cDNA library cell characterization Chem clones colloidal complex Coomassie blue detection differential digestion electroblotted Electrophoresis 18 electrospray electrospray ionization expression profiling Figure fluorescent fragment ion function gel images gene expression genetic genome genome sequencing glycan glycoproteins glycosylation Görg high-throughput Hochstrasser human hybridization immobilized ionization IPG strips Klose labeling MALDI MALDI-MS mapping mass spectrometry matching matrix-assisted laser membranes methods microarrays mixtures modifications molecular MPIs mRNA MS/MS patterns Patton peptide mass peptide mass fingerprinting pH gradients phage antibodies phosphopeptides phosphorylation polyacrylamide gels potential protein expression protein identification protein spots proteins separated proteome analysis proteomics PVDF quantitative Rabilloud reagents robot sample scanning sensitivity sequence databases sequence tags silver staining specific spectra spectrum SYPRO target techniques throughput tissue two-dimensional electrophoresis two-dimensional gel electrophoresis Yates