Real-time Analysis of Biomolecular Interactions: Applications of BIACORE

activity affinity constant amino acid antigen association and dissociation BIACORE system BIAevaluation binding Biol biological biomolecules biosensor biotinylated bulk effect calculation capture complex detection determined dextran dextran matrix dissociation constant dissociation phase dissociation rate constant epitope equation equilibrium evaluation factors fitting flow cell flow rate follistatin fragment function gene glycolipids GroEL human immobilized ligand inhibition injected InsP3 interac interaction k₁ kinetic analysis layer least squares lectin ligand lipid liposomes Mass Spectrometry mass transport membrane method mismatch molecular weight monoclonal antibodies mutations non-linear observed obtained oligonucleotides oligosaccharide parameters PCNA peptide phage displayed phospholipid probe protein purified rate constants reaction receptor refractive index regeneration residues response curves RPOINT running buffer sample screening sedimentation sensor chip surface sensor surface sensorgram signal soluble solution specific structure surface plasmon resonance Time(s tion ventral µg/ml