Real-time Analysis of Biomolecular Interactions: Applications of BIACORE |
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Page 99
... Time ( s ) 500 400 300 4000 ( ၁ ) ( d ) 1255A 3000 아 E256A 200 아 K254A 훈 2000- 1255A 1.1 X 10 - M 1000 100 E256A 1.3X10-9M K254A 3.0X10-9M 0 O 0 I -100 -1000 0 100 200 300 -50 Time ( s ) 50 50 Wild ( + ATP ) Wild ( -ATP ) D41A 150 ...
... Time ( s ) 500 400 300 4000 ( ၁ ) ( d ) 1255A 3000 아 E256A 200 아 K254A 훈 2000- 1255A 1.1 X 10 - M 1000 100 E256A 1.3X10-9M K254A 3.0X10-9M 0 O 0 I -100 -1000 0 100 200 300 -50 Time ( s ) 50 50 Wild ( + ATP ) Wild ( -ATP ) D41A 150 ...
Page 109
... is unknown . 2400 ( a ) 800 Activin ( b ) 700 BMP - 4 / 7 1900 600 500 BMP - 4 1400 BMP - 4 ( RU ) 400 300 900 200 400 100 BMP - 7 TGF - B 0 -100 -100 1 0 100 200 300 400 0 Time ( s ) 50 100 150 200 250 300 350 Time ( s ) 1000 ( c ) FS ...
... is unknown . 2400 ( a ) 800 Activin ( b ) 700 BMP - 4 / 7 1900 600 500 BMP - 4 1400 BMP - 4 ( RU ) 400 300 900 200 400 100 BMP - 7 TGF - B 0 -100 -100 1 0 100 200 300 400 0 Time ( s ) 50 100 150 200 250 300 350 Time ( s ) 1000 ( c ) FS ...
Page 192
... times improved affinity from a chain shuffled antibody fragment library that had undergone selection by panning , using ... Time ( s ) Fig . 7.8 . The dissociation phase of the interaction between immobilized antigen and soluble Fab frag ...
... times improved affinity from a chain shuffled antibody fragment library that had undergone selection by panning , using ... Time ( s ) Fig . 7.8 . The dissociation phase of the interaction between immobilized antigen and soluble Fab frag ...
Contents
Significance of the RealTime Analysis of Biological Interactions | 3 |
Principles of Surface Plasmon Resonance | 13 |
Principles of BIACORE | 23 |
Copyright | |
9 other sections not shown
Common terms and phrases
activity affinity constant amino acid antigen association and dissociation BIACORE system BIAevaluation binding Biol biological biomolecules biosensor biotinylated bulk effect calculation capture complex detection determined dextran dextran matrix dissociation constant dissociation phase dissociation rate constant epitope equation equilibrium evaluation factors fitting flow cell flow rate follistatin fragment function gene glycolipids GroEL human immobilized ligand inhibition injected InsP3 interac interaction k₁ kinetic analysis layer least squares lectin ligand lipid liposomes Mass Spectrometry mass transport membrane method mismatch molecular weight monoclonal antibodies mutations non-linear observed obtained oligonucleotides oligosaccharide parameters PCNA peptide phage displayed phospholipid probe protein purified rate constants reaction receptor refractive index regeneration residues response curves RPOINT running buffer sample screening sedimentation sensor chip surface sensor surface sensorgram signal soluble solution specific structure surface plasmon resonance Time(s tion ventral µg/ml