Real-time Analysis of Biomolecular Interactions: Applications of BIACORE |
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Page 121
... hybridoma was immobilized onto a BIACORE sensor chip by standard amine coupling . The affinity and kinetics of binding to soluble I - Ek loaded with variant MCC peptides that were measured and shown to differ dramatically in their ...
... hybridoma was immobilized onto a BIACORE sensor chip by standard amine coupling . The affinity and kinetics of binding to soluble I - Ek loaded with variant MCC peptides that were measured and shown to differ dramatically in their ...
Page 126
... hybridoma offered by a certain laboratory . But this hybridoma did not look very good and its growth rate was poor . Yet by persevering with cloning and culturing , it was eventually revived . So , 10 μl of the cultured supernatant was ...
... hybridoma offered by a certain laboratory . But this hybridoma did not look very good and its growth rate was poor . Yet by persevering with cloning and culturing , it was eventually revived . So , 10 μl of the cultured supernatant was ...
Page 206
... Hybridoma 15 : 343-350 4. Savage COS , Winearls C G , Jones S , Marshall P D , C.M. Lockwood ( 1987 ) Prospective study of radioimmunoassay for antibodies against neutrophil cytoplasm in diagnosis of systemic vasculitis . Lancet 20/6 ...
... Hybridoma 15 : 343-350 4. Savage COS , Winearls C G , Jones S , Marshall P D , C.M. Lockwood ( 1987 ) Prospective study of radioimmunoassay for antibodies against neutrophil cytoplasm in diagnosis of systemic vasculitis . Lancet 20/6 ...
Contents
Significance of the RealTime Analysis of Biological Interactions | 3 |
Principles of Surface Plasmon Resonance | 13 |
Principles of BIACORE | 23 |
Copyright | |
9 other sections not shown
Common terms and phrases
activity affinity constant amino acid antigen association and dissociation BIACORE system BIAevaluation binding Biol biological biomolecules biosensor biotinylated bulk effect calculation capture complex detection determined dextran dextran matrix dissociation constant dissociation phase dissociation rate constant epitope equation equilibrium evaluation factors fitting flow cell flow rate follistatin fragment function gene glycolipids GroEL human immobilized ligand inhibition injected InsP3 interac interaction k₁ kinetic analysis layer least squares lectin ligand lipid liposomes Mass Spectrometry mass transport membrane method mismatch molecular weight monoclonal antibodies mutations non-linear observed obtained oligonucleotides oligosaccharide parameters PCNA peptide phage displayed phospholipid probe protein purified rate constants reaction receptor refractive index regeneration residues response curves RPOINT running buffer sample screening sedimentation sensor chip surface sensor surface sensorgram signal soluble solution specific structure surface plasmon resonance Time(s tion ventral µg/ml