MAP Kinase Signaling Protocols

Front Cover
Rony Seger
Springer Science & Business Media, 2004 - Science - 332 pages
Mitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

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Contents

III
1
IV
29
V
49
VI
61
VII
89
VIII
97
IX
103
X
113
XV
177
XVI
203
XVII
211
XVIII
237
XIX
251
XX
263
XXI
283
XXII
299

XI
127
XII
145
XIII
155
XIV
167
XXIII
315
XXIV
323
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