Molecular and Cellular Methods in Developmental ToxicologyThis new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research. |
Contents
CXIX | 124 |
CXX | 125 |
CXXI | 127 |
CXXIII | 129 |
CXXVII | 130 |
CXXIX | 131 |
CXXX | 132 |
CXXXII | 133 |
| 12 | |
| 12 | |
| 12 | |
| 12 | |
| 12 | |
| 13 | |
XXV | 14 |
XXVII | 15 |
XXVIII | 16 |
| 17 | |
| 18 | |
XXXIV | 19 |
XXXV | 21 |
XXXVI | 22 |
XXXVIII | 23 |
XXXIX | 25 |
XL | 27 |
XLII | 28 |
XLIII | 29 |
XLIV | 30 |
XLV | 31 |
XLVI | 32 |
XLVII | 33 |
XLIX | 35 |
L | 36 |
LI | 37 |
LII | 38 |
LIII | 39 |
LIV | 40 |
LVI | 41 |
| 41 | |
LXI | 42 |
LXII | 43 |
LXIII | 45 |
LXIV | 47 |
LXVI | 48 |
LXVII | 48 |
LXVIII | 49 |
LXIX | 50 |
LXX | 51 |
LXXI | 52 |
LXXIII | 53 |
LXXIV | 55 |
| 59 | |
| 60 | |
LXXVII | 61 |
LXXVIII | 62 |
LXXIX | 64 |
LXXX | 67 |
LXXXI | 68 |
LXXXII | 69 |
LXXXIII | 70 |
LXXXVI | 71 |
LXXXVIII | 72 |
LXXXIX | 73 |
XC | 74 |
XCII | 78 |
| 83 | |
XCIV | 84 |
XCV | 85 |
XCVI | 85 |
XCVII | 87 |
XCVIII | 88 |
XCIX | 89 |
C | 90 |
CI | 95 |
CII | 99 |
CIII | 100 |
CIV | 104 |
CV | 113 |
| 115 | |
| 116 | |
CVIII | 117 |
CIX | 118 |
CX | 119 |
CXIII | 120 |
CXVI | 121 |
CXVII | 122 |
CXVIII | 123 |
CXXXIV | 135 |
CXXXV | 136 |
CXXXVI | 137 |
CXXXVII | 139 |
CXXXVIII | 140 |
CXXXIX | 141 |
CXL | 142 |
| 143 | |
| 144 | |
CXLIV | 145 |
CXLVII | 145 |
CXLIX | 146 |
CL | 147 |
CLII | 149 |
CLIII | 153 |
CLIV | 154 |
CLV | 156 |
CLVI | 157 |
CLVII | 158 |
CLVIII | 159 |
CLX | 160 |
CLXI | 165 |
CLXII | 165 |
CLXIII | 165 |
CLXV | 166 |
CLXVI | 167 |
| 169 | |
CLXIX | 170 |
CLXX | 171 |
CLXXI | 172 |
CLXXIII | 173 |
CLXXV | 174 |
CLXXVI | 175 |
CLXXVII | 176 |
| 177 | |
| 178 | |
CLXXXIV | 183 |
CLXXXVI | 184 |
CLXXXIX | 188 |
CXC | 189 |
CXCI | 192 |
CXCII | 193 |
CXCIII | 195 |
CXCIV | 197 |
CXCV | 199 |
CXCVI | 200 |
CXCVIII | 202 |
CCI | 203 |
CCII | 204 |
CCIII | 205 |
CCIV | 205 |
CCVII | 206 |
| 211 | |
| 212 | |
CCXI | 213 |
CCXIII | 215 |
CCXIV | 216 |
CCXV | 219 |
CCXVI | 221 |
CCXVII | 222 |
CCXVIII | 224 |
CCXIX | 225 |
CCXX | 228 |
CCXXI | 230 |
CCXXII | 231 |
CCXXIII | 232 |
CCXXV | 233 |
CCXXVI | 236 |
CCXXVII | 237 |
CCXXIX | 239 |
CCXXX | 240 |
CCXXXI | 241 |
CCXXXII | 242 |
CCXXXIV | 247 |
CCXXXV | 249 |
CCXXXVI | 253 |
CCXXXVII | 255 |
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Common terms and phrases
15 minutes 30 minutes acid agarose amplification analysis antigen antisense antisense ODNS apoptosis apoptotic aRNA assay beads binding Biology blastoderm block blot buffer cDNA cell death cellular chick containing cycle detection developmental diluted DNA polymerase electrophoresis embryos emulsion epitope ethanol EtOH Figure filter flow cytometry fluorescence gene expression genetic graft Höechst hybridization hybridoma immunohistochemistry Incubate injection labeling Laboratory membrane method mg/ml microinjection molecular molecules mouse embryos mRNA mutations NaCl neural tube Northern blot nuclei oligonucleotide overnight paraffin pellet pipette plasmid plates polymerase primary antibody primers probe procedure protein protocol reaction reagent remove restriction enzyme Rinse RNase room temperature saline sample secondary antibody sequence serum Sigma slides solution specific stage staining synthesis targeted techniques template teratogenic Teratology tissue sections transcription transfer transgenic transgenic mice vector wash Whole-Embryo Culture whole-mount zygote µg/ml