Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 31
Page 21
... camera lens ; 7 , cylinder lens ; 8 , photographic plate ; 9 , cell ; 10 , phase plate , showing diagonal phase border ; 11 , optical filter . lens forms an image of the light source slit on. 10. ULTRACENTRIFUGAL ANALYSIS 21.
... camera lens ; 7 , cylinder lens ; 8 , photographic plate ; 9 , cell ; 10 , phase plate , showing diagonal phase border ; 11 , optical filter . lens forms an image of the light source slit on. 10. ULTRACENTRIFUGAL ANALYSIS 21.
Page 23
... photographic plate ; 9 , Rayleigh slits ; 10 , double sector cell ; 11 , upper light limiting aperture ; 12 , Wratten 77A filter . apertures defining the duration of the period during which the. 10. ULTRACENTRIFUGAL ANALYSIS 23.
... photographic plate ; 9 , Rayleigh slits ; 10 , double sector cell ; 11 , upper light limiting aperture ; 12 , Wratten 77A filter . apertures defining the duration of the period during which the. 10. ULTRACENTRIFUGAL ANALYSIS 23.
Page 25
... filter or monochromator to the collimating lens where it is made parallel . The ultracentrifuge cell passes through the parallel light beam which is then condensed to give an image of the source slit . The subsequently diverging beam is ...
... filter or monochromator to the collimating lens where it is made parallel . The ultracentrifuge cell passes through the parallel light beam which is then condensed to give an image of the source slit . The subsequently diverging beam is ...
Page 26
... filter . requires a greater degree of operator sophistication than the photographic recording method . However , the method is very powerful , particularly for the study of interacting systems , since it enables measurements to be made ...
... filter . requires a greater degree of operator sophistication than the photographic recording method . However , the method is very powerful , particularly for the study of interacting systems , since it enables measurements to be made ...
Page 33
... filters . The instrument should also be capable of reasonable resolution . This may be checked by scanning a metal plate with a narrow slit milled in it , which should give rise to a rectangular trace with sharp shoulders . An ...
... filters . The instrument should also be capable of reasonable resolution . This may be checked by scanning a metal plate with a narrow slit milled in it , which should give rise to a rectangular trace with sharp shoulders . An ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero