Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 61
Page xiii
... Absorption John W. Donovan Fluorescence of Proteins Raymond F. Chen , Harold Edelhoch , and Robert F. Steiner Perturbation and Flow Techniques B. H. Havsteen Dielectric Properties of Proteins I. Dielectric Relaxation Shiro Takashima ...
... Absorption John W. Donovan Fluorescence of Proteins Raymond F. Chen , Harold Edelhoch , and Robert F. Steiner Perturbation and Flow Techniques B. H. Havsteen Dielectric Properties of Proteins I. Dielectric Relaxation Shiro Takashima ...
Page 18
... absorption optics . shows diagrammatically the most commonly available centerpieces . The multichannel cell , which allows observation of three solutions and three reference solvents at once , does not have sectorial channels because ...
... absorption optics . shows diagrammatically the most commonly available centerpieces . The multichannel cell , which allows observation of three solutions and three reference solvents at once , does not have sectorial channels because ...
Page 19
... absorption optical systems to give separate images of the light source slit in a plane near the camera lens . The image at the plane of the photographic plate is not shifted , but the absorption pattern for a particular cell is selected ...
... absorption optical systems to give separate images of the light source slit in a plane near the camera lens . The image at the plane of the photographic plate is not shifted , but the absorption pattern for a particular cell is selected ...
Page 25
... Absorption . The high sensitivity and specificity of spectro- photometric methods suggest that they should be suitable for the deter- mination of concentrations in an ultracentrifuge cell . A diagram of an absorption optical system is ...
... Absorption . The high sensitivity and specificity of spectro- photometric methods suggest that they should be suitable for the deter- mination of concentrations in an ultracentrifuge cell . A diagram of an absorption optical system is ...
Page 26
... absorption optical system of the Beckman / Spinco ultracentrifuge . The plateau region is shown cross - hatched . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ...
... absorption optical system of the Beckman / Spinco ultracentrifuge . The plateau region is shown cross - hatched . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero