Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 64
Page 3
... band function defined by Eq . ( 64 ) activity isothermal compressibility 1. Introduction Ultracentrifugal analysis by the sedimentation velocity technique is a powerful method for the characterization of proteins since it is capable of ...
... band function defined by Eq . ( 64 ) activity isothermal compressibility 1. Introduction Ultracentrifugal analysis by the sedimentation velocity technique is a powerful method for the characterization of proteins since it is capable of ...
Page 20
... band- forming cell ; the protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window ...
... band- forming cell ; the protein solution layers through the capillary on the face of the centerpiece . ( e ) Type II band - forming centerpiece ; the protein solution layers through a narrow gap between the centerpiece and the window ...
Page 28
... band of solution less dense than the lightest solvent is placed on top of the solvent gradient . The sedimentation constants obtained from such experiments must be corrected for the density and viscosity of the solvent down the tube ...
... band of solution less dense than the lightest solvent is placed on top of the solvent gradient . The sedimentation constants obtained from such experiments must be corrected for the density and viscosity of the solvent down the tube ...
Page 69
... band or zone of protein sediments into solvent rather than solution ( Fig . 26 ) . If a thin layer or lamella of solution is placed on top of a layer of pure solvent in a centrifugal field , convection will immediately occur because of ...
... band or zone of protein sediments into solvent rather than solution ( Fig . 26 ) . If a thin layer or lamella of solution is placed on top of a layer of pure solvent in a centrifugal field , convection will immediately occur because of ...
Page 72
... band is to be followed with precision . 3. Concentration of the Stabilizing Solute The considerations governing the stability of solute zones and the fac- tors governing the diffusion gradient and field gradient of the stabilizing 1.0 E ...
... band is to be followed with precision . 3. Concentration of the Stabilizing Solute The considerations governing the stability of solute zones and the fac- tors governing the diffusion gradient and field gradient of the stabilizing 1.0 E ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero