Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Results 1-5 of 91
Page 6
... Equation For a two - component system the rate of change of concentration ( c1 ) of the solute with time at any point in the ultracentrifuge cell at a dis- tance x from the center of rotation is given by the ultracentrifuge differ ...
... Equation For a two - component system the rate of change of concentration ( c1 ) of the solute with time at any point in the ultracentrifuge cell at a dis- tance x from the center of rotation is given by the ultracentrifuge differ ...
Page 7
... be independent of concentration and any other factors , an equation describing the time dependence of the plateau region concentration , ob- served in sedimentation velocity , can be obtained . In. 10. ULTRACENTRIFUGAL ANALYSIS 7.
... be independent of concentration and any other factors , an equation describing the time dependence of the plateau region concentration , ob- served in sedimentation velocity , can be obtained . In. 10. ULTRACENTRIFUGAL ANALYSIS 7.
Page 8
... Equation ( 4 ) characterizes the sectorial dilu- tion of the plateau region shown in Fig . 2 . The integration of the differential equation to describe the shape of a sedimenting boundary , that is , the zone between the zero ...
... Equation ( 4 ) characterizes the sectorial dilu- tion of the plateau region shown in Fig . 2 . The integration of the differential equation to describe the shape of a sedimenting boundary , that is , the zone between the zero ...
Page 11
... equation describing the con- centration distribution for a single solute at equilibrium is as follows w2xC1 dci M1 ( 1 Up ) XC 1 = dx ln yı -RFP ) w2ze [ 1 + a ( @In M ) , ] ᎡᎢ T.P. 1 ( 5 ) C dc dx ( a ) ; dc dx ( 10. ULTRACENTRIFUGAL ...
... equation describing the con- centration distribution for a single solute at equilibrium is as follows w2xC1 dci M1 ( 1 Up ) XC 1 = dx ln yı -RFP ) w2ze [ 1 + a ( @In M ) , ] ᎡᎢ T.P. 1 ( 5 ) C dc dx ( a ) ; dc dx ( 10. ULTRACENTRIFUGAL ...
Page 12
... equation applies if the solution is incompressible , an approximation that is valid for protein solution . y1 is the activity coefficient of the solute on the c scale ( mass per unit volume ) . The chemical potential of component 1 , μ1 ...
... equation applies if the solution is incompressible , an approximation that is valid for protein solution . y1 is the activity coefficient of the solute on the c scale ( mass per unit volume ) . The chemical potential of component 1 , μ1 ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero