Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Page 2
... fringe number light path length j λ wavelength of light b optical lever arm F vertical magnification factor G horizontal magnification factor E enlarger magnification factor 0 schlieren bar angle A area under schlieren peak Y X S height ...
... fringe number light path length j λ wavelength of light b optical lever arm F vertical magnification factor G horizontal magnification factor E enlarger magnification factor 0 schlieren bar angle A area under schlieren peak Y X S height ...
Page 19
... fringes for measurement purposes . Wedge window cells that deflect the emergent light beam about a radius through the center of rotation lying in the plane of the upper window are called left or right - side wedge windows . They are ...
... fringes for measurement purposes . Wedge window cells that deflect the emergent light beam about a radius through the center of rotation lying in the plane of the upper window are called left or right - side wedge windows . They are ...
Page 24
... fringes ; 2 , outer reference fringes ; 3 , reference wire image ( used to determine accurate x distances ) ; 4 , air fringes ; 5 , air - solution meniscus and air- solvent meniscus ; 6 , solution - oil meniscus . ( b ) 1 - mm column ...
... fringes ; 2 , outer reference fringes ; 3 , reference wire image ( used to determine accurate x distances ) ; 4 , air fringes ; 5 , air - solution meniscus and air- solvent meniscus ; 6 , solution - oil meniscus . ( b ) 1 - mm column ...
Page 25
... fringes flanking the pattern generated by the cell . For the determination of the concentration at a given level in the ... fringe pattern is shown in Fig . 11. Detailed procedures for the alignment of an ultracentrifuge op- tical system ...
... fringes flanking the pattern generated by the cell . For the determination of the concentration at a given level in the ... fringe pattern is shown in Fig . 11. Detailed procedures for the alignment of an ultracentrifuge op- tical system ...
Page 30
... used for measuring the photograph . b . The Measurement of Rayleigh Interference Patterns . The Rayleigh interference system records concentration distributions within the cell in terms of interference fringes ( Fig . 11 ) 30 J. H. COATES.
... used for measuring the photograph . b . The Measurement of Rayleigh Interference Patterns . The Rayleigh interference system records concentration distributions within the cell in terms of interference fringes ( Fig . 11 ) 30 J. H. COATES.
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero