Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 41
Page 2
... Density Gradient 80 X. A Brief Comparison of Some Commercially Available ... density of solution μ chemical potential R T y B M w Δη gas constant temperature ... optical lever arm F vertical magnification factor G horizontal magnification ...
... Density Gradient 80 X. A Brief Comparison of Some Commercially Available ... density of solution μ chemical potential R T y B M w Δη gas constant temperature ... optical lever arm F vertical magnification factor G horizontal magnification ...
Page 4
... density gradient . Isopycnic density gradient methods have been widely used for the char- acterization of nucleic ... optical system of the ultracentrifuge to allow for the registration of inverted peaks by elevating the cylindrical lens ...
... density gradient . Isopycnic density gradient methods have been widely used for the char- acterization of nucleic ... optical system of the ultracentrifuge to allow for the registration of inverted peaks by elevating the cylindrical lens ...
Page 17
... density increment is measured . Furthermore , if refractive index measurements are used to determine the protein ... optical means , the parallel sides of the cavity are made by sandwiching on either side of the centerpiece , windows which ...
... density increment is measured . Furthermore , if refractive index measurements are used to determine the protein ... optical means , the parallel sides of the cavity are made by sandwiching on either side of the centerpiece , windows which ...
Page 19
... optical system . In isopycnic density gradient experiments where steep gradients in the concentration of the solvent are encountered , the refractive effect of the gradient may deflect the light beam so extensively that it hits the side ...
... optical system . In isopycnic density gradient experiments where steep gradients in the concentration of the solvent are encountered , the refractive effect of the gradient may deflect the light beam so extensively that it hits the side ...
Page 25
... optical system is about one order of magnitude greater than the schlieren ... optical system is shown in Fig . 12. Light from a mercury or xenon lamp passes ... density versus distance down the cell directly . Double sector cells may be ...
... optical system is about one order of magnitude greater than the schlieren ... optical system is shown in Fig . 12. Light from a mercury or xenon lamp passes ... density versus distance down the cell directly . Double sector cells may be ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero