Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 60
Page 2
... angle A area under schlieren peak Y X S height of schlieren trace distance measured on photographic plate in x direction square root of residual variance 59955 91 b slope of a straight line N S.E. t η 2 J. H. COATES Glossary of Symbols.
... angle A area under schlieren peak Y X S height of schlieren trace distance measured on photographic plate in x direction square root of residual variance 59955 91 b slope of a straight line N S.E. t η 2 J. H. COATES Glossary of Symbols.
Page 4
... peaks by elevating the cylindrical lens . In this chapter we attempt a description of the main methods of sedi- mentation analysis without detailed justification of their theoretical basis . Fundamental ideas concerning the ...
... peaks by elevating the cylindrical lens . In this chapter we attempt a description of the main methods of sedi- mentation analysis without detailed justification of their theoretical basis . Fundamental ideas concerning the ...
Page 9
... peaks . 3. Multicomponent Systems : Solvent and Two or More Solutes Consider a hypothetical solution containing two uncharged noninter- acting protein solutes . If their sedimentation coefficients are independent of concentration , the ...
... peaks . 3. Multicomponent Systems : Solvent and Two or More Solutes Consider a hypothetical solution containing two uncharged noninter- acting protein solutes . If their sedimentation coefficients are independent of concentration , the ...
Page 10
... peak is decreased while that of the following peak is increased ; for explanation see text . component noninteracting systems , an additional correction ( Schachman , 1959 , p . 116 ) must be made for the Johnston - Ogston effect ...
... peak is decreased while that of the following peak is increased ; for explanation see text . component noninteracting systems , an additional correction ( Schachman , 1959 , p . 116 ) must be made for the Johnston - Ogston effect ...
Page 23
... peak is shown in Fig . 9 . b . Rayleigh Interference . Usually the schlieren optical system of the ultracentrifuge has only to be slightly modified to enable Rayleigh inter- ferograms to be obtained . The essential components of the ...
... peak is shown in Fig . 9 . b . Rayleigh Interference . Usually the schlieren optical system of the ultracentrifuge has only to be slightly modified to enable Rayleigh inter- ferograms to be obtained . The essential components of the ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero