Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Page vii
... reflected in much of the material in this volume and the one to follow . This volume follows the pattern set by Part A of this treatise . Physical techniques which are well established in protein chemistry are described in a detailed ...
... reflected in much of the material in this volume and the one to follow . This volume follows the pattern set by Part A of this treatise . Physical techniques which are well established in protein chemistry are described in a detailed ...
Page 54
... reflect their nonideality rather than their molecular weight . Because the exact form of expressions for extrapolation cannot be explicitly stated when all of the solute virial coefficients depend on all the solute concen- trations ...
... reflect their nonideality rather than their molecular weight . Because the exact form of expressions for extrapolation cannot be explicitly stated when all of the solute virial coefficients depend on all the solute concen- trations ...
Page 61
... reflects precisely the rate of movement of the solute in the plateau region . When the plateau contains several ... reflected in the weight - average sedimentation coefficient . For example , if a solute can undergo self - association by ...
... reflects precisely the rate of movement of the solute in the plateau region . When the plateau contains several ... reflected in the weight - average sedimentation coefficient . For example , if a solute can undergo self - association by ...
Page 68
... reflect properties of the monomer species . If data concerning the stoichiometry of association and the equilibrium constants are required , measurements of apparent molecular weights at finite con- centration must be made . Adams and ...
... reflect properties of the monomer species . If data concerning the stoichiometry of association and the equilibrium constants are required , measurements of apparent molecular weights at finite con- centration must be made . Adams and ...
Page 171
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Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero