Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 49
Page 10
... reactions occur between solutes , the interpreta- tion of sedimentation velocity data can be complicated . Interacting sys- tems are discussed in Section VII . Many proteins are studied under conditions where they carry an elec- tric ...
... reactions occur between solutes , the interpreta- tion of sedimentation velocity data can be complicated . Interacting sys- tems are discussed in Section VII . Many proteins are studied under conditions where they carry an elec- tric ...
Page 60
... reaction of the reaction being studied is not zero , since the equilibrium constant for the reaction will in these circumstances be a function of pressure . In a conventional ana- lytical ultracentrifuge experiment the pressure at the ...
... reaction of the reaction being studied is not zero , since the equilibrium constant for the reaction will in these circumstances be a function of pressure . In a conventional ana- lytical ultracentrifuge experiment the pressure at the ...
Page 61
... reactions and transport processes . If the appropriate partial differential equations are set up , it appears that analytical solutions can only be readily derived for the limiting cases of very fast or very slow reaction rates ...
... reactions and transport processes . If the appropriate partial differential equations are set up , it appears that analytical solutions can only be readily derived for the limiting cases of very fast or very slow reaction rates ...
Page 64
... reaction . Both monomer and associated species occur throughout the whole of the reaction boundary . Where it is suspected that a complex boundary is a reaction boundary , it is worthwhile carry- ing out duplicate sedimentation ...
... reaction . Both monomer and associated species occur throughout the whole of the reaction boundary . Where it is suspected that a complex boundary is a reaction boundary , it is worthwhile carry- ing out duplicate sedimentation ...
Page 65
... reaction of the type A + B C is occurring , have been calculated both by means of continuity equations and the use of constituent concepts . The original papers ( Gilbert and Jenkins , 1959 ; Nichol and Ogston , 1965a ) should be ...
... reaction of the type A + B C is occurring , have been calculated both by means of continuity equations and the use of constituent concepts . The original papers ( Gilbert and Jenkins , 1959 ; Nichol and Ogston , 1965a ) should be ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero