Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Page 2
... refractive index increment fringe number light path length j λ wavelength of light b optical lever arm F vertical magnification factor G horizontal magnification factor E enlarger magnification factor 0 schlieren bar angle A area under ...
... refractive index increment fringe number light path length j λ wavelength of light b optical lever arm F vertical magnification factor G horizontal magnification factor E enlarger magnification factor 0 schlieren bar angle A area under ...
Page 3
... refractive index using sodium light at 25 ° C length of solution column square root of the variance of sedimenting band function defined by Eq . ( 64 ) activity isothermal compressibility 1. Introduction Ultracentrifugal analysis by the ...
... refractive index using sodium light at 25 ° C length of solution column square root of the variance of sedimenting band function defined by Eq . ( 64 ) activity isothermal compressibility 1. Introduction Ultracentrifugal analysis by the ...
Page 4
... refractive index gradients produced by flotation are negative and it is usual to adjust the schlieren optical system of the ultracentrifuge to allow for the registration of inverted peaks by elevating the cylindrical lens . In this ...
... refractive index gradients produced by flotation are negative and it is usual to adjust the schlieren optical system of the ultracentrifuge to allow for the registration of inverted peaks by elevating the cylindrical lens . In this ...
Page 15
... refractive index ) which is proportional to the weight concentration c1 . Equation ( 10 ) as it stands is thermodynamically exact , assuming that higher terms in the virial expansion may be neglected . If all the quan- tities are ...
... refractive index ) which is proportional to the weight concentration c1 . Equation ( 10 ) as it stands is thermodynamically exact , assuming that higher terms in the virial expansion may be neglected . If all the quan- tities are ...
Page 17
... refractive index measurements are used to determine the protein concentration in the cell , error will occur if , due to interaction , the low molecular weight electrolyte distributes very differently in the presence of the protein . D ...
... refractive index measurements are used to determine the protein concentration in the cell , error will occur if , due to interaction , the low molecular weight electrolyte distributes very differently in the presence of the protein . D ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero