Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 49
Page 2
... rotation concentration angular velocity of rotation time from effective start of sedimentation v partial specific volume P density of solution μ chemical potential R T y B M w Δη gas constant temperature activity coefficient virial ...
... rotation concentration angular velocity of rotation time from effective start of sedimentation v partial specific volume P density of solution μ chemical potential R T y B M w Δη gas constant temperature activity coefficient virial ...
Page 5
... rotation is shown in Fig . 1. It is usual for the solutions to have an air bubble on the centripetal side as shown in Fig . 1. As the sedi- menting material cannot pass through the cell bottom ( centrifugal side ) , a layer of ...
... rotation is shown in Fig . 1. It is usual for the solutions to have an air bubble on the centripetal side as shown in Fig . 1. As the sedi- menting material cannot pass through the cell bottom ( centrifugal side ) , a layer of ...
Page 6
... rotation of the rotor . Key : 1 , axis of rotation ; 2 , air bubble ; 3 , protein solution ; 4 , fluorocarbon oil ; 5 , direction of light rays . mentation rate greatly exceeds the rate of diffusion from regions of high to low solute ...
... rotation of the rotor . Key : 1 , axis of rotation ; 2 , air bubble ; 3 , protein solution ; 4 , fluorocarbon oil ; 5 , direction of light rays . mentation rate greatly exceeds the rate of diffusion from regions of high to low solute ...
Page 11
... rotation is of the same order as the rate of diffusion of molecules from high concentration regions near the cell bottom to the lower concentration regions nearer the center of rotation . Under these conditions the concentration of ...
... rotation is of the same order as the rate of diffusion of molecules from high concentration regions near the cell bottom to the lower concentration regions nearer the center of rotation . Under these conditions the concentration of ...
Page 17
... rotation of the rotor . The cavity is ma- chined out of a solid centerpiece . Since the sedimentation is usually ob- served by optical means , the parallel sides of the cavity are made by sandwiching on either side of the centerpiece ...
... rotation of the rotor . The cavity is ma- chined out of a solid centerpiece . Since the sedimentation is usually ob- served by optical means , the parallel sides of the cavity are made by sandwiching on either side of the centerpiece ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero