Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 47
Page 1
... Sedimentation Velocity Behavior of Noninteracting Solutes . A. Requirements that Must Be Met for a Satisfactory Use of the Method B. Determination of Sedimentation Coefficients C. Detection of Heterogeneity . 37 37 37 38 42 D ...
... Sedimentation Velocity Behavior of Noninteracting Solutes . A. Requirements that Must Be Met for a Satisfactory Use of the Method B. Determination of Sedimentation Coefficients C. Detection of Heterogeneity . 37 37 37 38 42 D ...
Page 2
... Sedimentation Coefficients IX . Isopycnic Density Gradient Sedimentation A. Introduction 0 60 588 61 69 69 72 75 78 78 22888 B. Sedimentation Equilibrium in a Buoyant Density Gradient 80 X. A Brief Comparison of Some Commercially ...
... Sedimentation Coefficients IX . Isopycnic Density Gradient Sedimentation A. Introduction 0 60 588 61 69 69 72 75 78 78 22888 B. Sedimentation Equilibrium in a Buoyant Density Gradient 80 X. A Brief Comparison of Some Commercially ...
Page 3
... sedimentation coefficient vs. concentration plot frictional coefficient Avogadro's number radius of hydrodynamic particle 2D / ( xa ) 2w2s rate constant degree of association rate of movement of a component in an interacting mixture ...
... sedimentation coefficient vs. concentration plot frictional coefficient Avogadro's number radius of hydrodynamic particle 2D / ( xa ) 2w2s rate constant degree of association rate of movement of a component in an interacting mixture ...
Page 5
... sedimentation velocity experiment a protein solution is subjected to a centrifugal field of about 250,000 g ... coefficient of a solute ; that is , the ob- served sedimentation velocity divided by the centrifugal acceleration that ...
... sedimentation velocity experiment a protein solution is subjected to a centrifugal field of about 250,000 g ... coefficient of a solute ; that is , the ob- served sedimentation velocity divided by the centrifugal acceleration that ...
Page 6
... sedimentation coefficient and diffusion coefficient , respec- tively , of the solute component , component ( 1 ) . w is the angular velocity of the rotor . This equation , solved with appropriate boundary conditions , can in principle ...
... sedimentation coefficient and diffusion coefficient , respec- tively , of the solute component , component ( 1 ) . w is the angular velocity of the rotor . This equation , solved with appropriate boundary conditions , can in principle ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero