Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 1-5 of 35
Page 19
... slit in a plane near the camera lens . The image at the plane of the photographic plate is not shifted , but the absorption pattern for a particular cell is selected by appropriate masking in the plane of the source slit image . A ...
... slit in a plane near the camera lens . The image at the plane of the photographic plate is not shifted , but the absorption pattern for a particular cell is selected by appropriate masking in the plane of the source slit image . A ...
Page 21
... slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 , camera lens ; 7 , cylinder lens ; 8 , photographic plate ; 9 , cell ; 10 , phase plate , showing diagonal phase border ; 11 , optical filter . lens ...
... slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 , camera lens ; 7 , cylinder lens ; 8 , photographic plate ; 9 , cell ; 10 , phase plate , showing diagonal phase border ; 11 , optical filter . lens ...
Page 22
... slit is imaged in the plane of the phase plate , the refracted rays form images of the source slit displaced to the rear of the optical system . The displacement from the image of the source slit produced by nonrefracted rays is ...
... slit is imaged in the plane of the phase plate , the refracted rays form images of the source slit displaced to the rear of the optical system . The displacement from the image of the source slit produced by nonrefracted rays is ...
Page 23
... slits incorporated in the lower window holder . Each slit allows a narrow beam of rays to pass through one of the sectors of the cell . Immediately above the cell and attached to the condensing lens mount are a pair of wider slits that ...
... slits incorporated in the lower window holder . Each slit allows a narrow beam of rays to pass through one of the sectors of the cell . Immediately above the cell and attached to the condensing lens mount are a pair of wider slits that ...
Page 24
... slit so that an interference pattern is formed in the plane of the photographic plate . The phase plate is rotated ... slits must be symmetrical about. 24 J. H. COATES.
... slit so that an interference pattern is formed in the plane of the photographic plate . The phase plate is rotated ... slits must be symmetrical about. 24 J. H. COATES.
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero