Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 50
Page 1-6
... antibiotics is believed to interfere with their active transport into the cell ( for review , see Davies and Smith 1978 ) . Virtually all plasmid vectors in common use carry one or more of the antibiotic resistance genes described above ...
... antibiotics is believed to interfere with their active transport into the cell ( for review , see Davies and Smith 1978 ) . Virtually all plasmid vectors in common use carry one or more of the antibiotic resistance genes described above ...
Page 1-81
... antibiotic resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently agitated ( 225 cycles / minute or less ) during the recovery period . 16. Transfer the appropriate volume ...
... antibiotic resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently agitated ( 225 cycles / minute or less ) during the recovery period . 16. Transfer the appropriate volume ...
Page 1-87
... antibiotic resistance genes and an appropriate distribution of restriction enzyme cleavage sites . The DNA to be inserted and the purified plasmid DNA are digested with restriction enzymes that recognize sites located in only one of the ...
... antibiotic resistance genes and an appropriate distribution of restriction enzyme cleavage sites . The DNA to be inserted and the purified plasmid DNA are digested with restriction enzymes that recognize sites located in only one of the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml