Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-95
... extracts prepared from bacteria infected with bacteriophage à mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
... extracts prepared from bacteria infected with bacteriophage à mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
Page 2-98
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
Page 2-104
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris · Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris · Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml