Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-67
... 37 ° C to allow the bacteriophage particles to adsorb . 3. Add 4 ml of NZCYM or LB medium and incubate with vigorous shaking until lysis occurs ( 6-12 hours at 37 ° C ) . Shaking can be best accomplished by positioning the tube in a ...
... 37 ° C to allow the bacteriophage particles to adsorb . 3. Add 4 ml of NZCYM or LB medium and incubate with vigorous shaking until lysis occurs ( 6-12 hours at 37 ° C ) . Shaking can be best accomplished by positioning the tube in a ...
Page 4-48
... hours at 37 ° C with strong agitation ( 300 cycles / minute ) . The bacterial culture should be only slightly turbid at this stage . If growth is too vigorous , dilute the culture with prewarmed 2 × YT medium until the turbidity is only ...
... hours at 37 ° C with strong agitation ( 300 cycles / minute ) . The bacterial culture should be only slightly turbid at this stage . If growth is too vigorous , dilute the culture with prewarmed 2 × YT medium until the turbidity is only ...
Page 6-55
... C . Wash the cell pellet twice with 0.05 M EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at ... hours at 37 ° C in 50 volumes of 0.5 м EDTA ( pH 8.0 ) , 0.01 м Tris Cl ( pH 7.6 ) containing 1 % B ...
... C . Wash the cell pellet twice with 0.05 M EDTA ( pH 8.0 ) , 0.01 M Tris Cl ( pH 7.6 ) . . 2. Resuspend the cells at ... hours at 37 ° C in 50 volumes of 0.5 м EDTA ( pH 8.0 ) , 0.01 м Tris Cl ( pH 7.6 ) containing 1 % B ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml