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plasmids and bacteriophages
Purification of DNA from living cells
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adaptor amino acid ampicillin animal antibiotic antibody bacterial cells bacteriophage bacterium base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleave cloned gene cloning vectors cloning vehicle coded coli colonies complementary contain copy number cosmid culture density digest DNA fragments DNA sequencing double-stranded EcoRI EcoRl electrophoresis enzyme example expression vector Figure gene cloning gene cloning experiment genomic library host cell hybridization probing infection cycle Insertional inactivation insulin intron lacZ ligase ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid plasmid DNA polylinker polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein removed replication restriction endonucleases restriction sites resulting segment selectable marker single-stranded specific sticky ends strand structure synthesis techniques tetracycline transformed translation product types unique restriction virus vitro yeast zein