Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 108
... genomic libraries to be constructed The main use of all λ - based vectors is to carry DNA fragments that are too large to be ... genomic library . A genomic library is a set of recombinant clones that contain all of the DNA present in an ...
... genomic libraries to be constructed The main use of all λ - based vectors is to carry DNA fragments that are too large to be ... genomic library . A genomic library is a set of recombinant clones that contain all of the DNA present in an ...
Page 135
... genomic library ( see p . 108 ) is a collection of clones sufficient in number to be likely to contain every single gene present in a parti- cular organism . Genomic libraries are prepared by purifying total cell DNA , and then making a ...
... genomic library ( see p . 108 ) is a collection of clones sufficient in number to be likely to contain every single gene present in a parti- cular organism . Genomic libraries are prepared by purifying total cell DNA , and then making a ...
Page 146
... cDNA clone , identified earlier in the chapter by abundancy probing , is used to probe a genomic library , then it will hybridize not only to its own gene , but to a variety of other genes as well ( Fig . 8.14c ) . These will all be ...
... cDNA clone , identified earlier in the chapter by abundancy probing , is used to probe a genomic library , then it will hybridize not only to its own gene , but to a variety of other genes as well ( Fig . 8.14c ) . These will all be ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein