Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 41
... Purification of DNA from λ phage particles Deproteinization of the redissolved PEG precipitate is sometimes sufficient ... purified by following the standard procedures for plasmid preparation . A cell extract is prepared from cells 1.35 ...
... Purification of DNA from λ phage particles Deproteinization of the redissolved PEG precipitate is sometimes sufficient ... purified by following the standard procedures for plasmid preparation . A cell extract is prepared from cells 1.35 ...
Page 43
... purified enzymes . Within the cell these enzymes participate in essential processes such as DNA replication and transcription , breakdown of unwanted or foreign DNA ( for example , invading virus DNA ) , repair of mutated DNA , and ...
... purified enzymes . Within the cell these enzymes participate in essential processes such as DNA replication and transcription , breakdown of unwanted or foreign DNA ( for example , invading virus DNA ) , repair of mutated DNA , and ...
Page 147
... Purified antibody Figure 8.15 Antibodies . ( a ) Antibodies in the bloodstream bind to foreign molecules and help degrade them . ( b ) Purified antibodies can be obtained from a small volume of blood taken from a rabbit injected with ...
... Purified antibody Figure 8.15 Antibodies . ( a ) Antibodies in the bloodstream bind to foreign molecules and help degrade them . ( b ) Purified antibodies can be obtained from a small volume of blood taken from a rabbit injected with ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein