PCR Cloning Protocols

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Bing-Yuan Chen, Harry W. Janes
Springer Science & Business Media, 2002 - Science - 439 pages
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

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very useful and helpful! nowadays people can buy products and kits, but I think it is important to understand how such kits work, particularly important to people who work on method development.

Contents

III
3
IV
19
V
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VI
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VII
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VIII
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IX
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X
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XXVII
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XXVIII
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XXIX
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XXX
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XXXI
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XXXII
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XXXIII
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XXXIV
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XI
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XII
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XIII
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XIV
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XV
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XVI
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XVII
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XVIII
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XIX
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XX
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XXI
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XXII
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XXIII
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XXIV
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XXV
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XXVI
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XXXV
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XXXVI
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XXXVII
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XXXVIII
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XXXIX
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XL
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XLI
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XLII
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XLIII
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XLIV
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XLV
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XLVI
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XLVII
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XLVIII
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