Protein Protease Inhibitor: The Case of Streptomyces Subtilisin Inhibitor (SSI)

a-chymotrypsin absorption Akasaka alkaline protease amino acid amino acid residues atoms binding of SSI Biochem Biol BPTI C-terminal carbonyl carbonyl carbon Chem chemical shift chymotrypsin concentration conformation contact region crystal structure crystallographic curve D₂O denaturation difference dimer dissociation disulfide electron density enzyme equilibrium exchange fluorescence H NMR Hiromi hydrogen bond Ikenaka inhibitory activity Inouye interaction k₁ Komiyama magnetic measured method methyl Mitsui molecular weight molecule observed obtained ovomucoid peak peptide bond peptide NH plasminostreptin protease inhibitor protein protease inhibitors protons Raman rate constant reaction reactive resonance rotation Sato & Murao scissile scissile bond serine proteases shown in Fig side chain signal solution solvent spectra ẞ-sheet SSI-subtilisin BPN stopped-flow Streptomyces subtilisin inhibitor substrate subtilisin BPN subunit temperature tertiary structure titration Tonomura transition trypsin tryptophan Uehara values wavelength X-ray