Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 15
Page 1578
... DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 ... Ligase 5.62 E. coli DNA Ligase 5.64 Bacteriophage T4 RNA Ligase 5.66 Bacteriophage T4 Polynucleotide Kinase 5.68 Alkaline ...
... DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 ... Ligase 5.62 E. coli DNA Ligase 5.64 Bacteriophage T4 RNA Ligase 5.66 Bacteriophage T4 Polynucleotide Kinase 5.68 Alkaline ...
Page F-7
... DNA ligase mixture is prepared as follows : · 1 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 200 mм dithiothreitol 10 mM ATP H2O 1.0 με 1.0 με 1.0 με 1.0 με 5.5 με bacteriophage T4 DNA ligase 1 Weiss unit Mix the components in a tube stored in an ...
... DNA ligase mixture is prepared as follows : · 1 M Tris Cl ( pH 7.6 ) 100 mM MgCl2 200 mм dithiothreitol 10 mM ATP H2O 1.0 με 1.0 με 1.0 με 1.0 με 5.5 με bacteriophage T4 DNA ligase 1 Weiss unit Mix the components in a tube stored in an ...
Page F-9
... DNA 0.1-0.5 με ( in a volume of 7 μl or less ) 1-2 με phosphorylated linkers ( in a volume of 8 μl or less ) H2O to 15 μl 5 mM ATP 2 μl 2 μl 1 μl 10 blunt - end ligation buffer bacteriophage T4 DNA ligase ( 1-2 Weiss units ) Incubate ...
... DNA 0.1-0.5 με ( in a volume of 7 μl or less ) 1-2 με phosphorylated linkers ( in a volume of 8 μl or less ) H2O to 15 μl 5 mM ATP 2 μl 2 μl 1 μl 10 blunt - end ligation buffer bacteriophage T4 DNA ligase ( 1-2 Weiss units ) Incubate ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml