Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1585
... DNA TEMPLATES BY BACTERIOPHAGE DNA - DEPENDENT RNA POLYMERASES 10.27 Plasmid Vectors for Preparing RNA Probes 10.29 ... MOLECULES WITH PROTRUDING 5 ' TERMINI AS SUBSTRATES 10.60 USING DNA MOLECULES WITH BLUNT ENDS OR RECESSED 5 ' TERMINI ...
... DNA TEMPLATES BY BACTERIOPHAGE DNA - DEPENDENT RNA POLYMERASES 10.27 Plasmid Vectors for Preparing RNA Probes 10.29 ... MOLECULES WITH PROTRUDING 5 ' TERMINI AS SUBSTRATES 10.60 USING DNA MOLECULES WITH BLUNT ENDS OR RECESSED 5 ' TERMINI ...
Page 1654
... DNA molecules into eukaryotic cells by electropora- tion , see Andreason and Evans [ 1988 ] . For a discussion of the use of electroporation to introduce DNA into bacterial cells , see Chapter 1 , page 1.74 . ) The procedure has been ...
... DNA molecules into eukaryotic cells by electropora- tion , see Andreason and Evans [ 1988 ] . For a discussion of the use of electroporation to introduce DNA into bacterial cells , see Chapter 1 , page 1.74 . ) The procedure has been ...
Page E-4
... DNA ( see Note below ) . Note The organic and aqueous phases may be mixed by vortexing when isolating small DNA molecules ( < 10 kb ) or by gentle shaking when isolating DNA molecules of moderate size ( 10-30 kb ) . When isolating large DNA ...
... DNA ( see Note below ) . Note The organic and aqueous phases may be mixed by vortexing when isolating small DNA molecules ( < 10 kb ) or by gentle shaking when isolating DNA molecules of moderate size ( 10-30 kb ) . When isolating large DNA ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml