Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1649
... EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . • 5. Slowly add 1 ml of ice - cold 50 mM Tris Cl ( pH 8.0 ) , and incubate the suspension for 15 minutes at 37 ° C . The bacterial cell wall is digested during this incubation . The ...
... EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . • 5. Slowly add 1 ml of ice - cold 50 mM Tris Cl ( pH 8.0 ) , and incubate the suspension for 15 minutes at 37 ° C . The bacterial cell wall is digested during this incubation . The ...
Page B-11
... Phosphate - buffered saline. 1 M Dithiothreitol ( DTT ) 0.5 M EDTA ( pH 8.0 ) Ethidium bromide ( 10 mg / ml ) 2 × HEPES - buffered saline IPTG -20 ° C . Dissolve 3.09 g of DTT in 20 ml of 0.01 M sodium acetate ( pH 5.2 ) . Sterilize by ...
... Phosphate - buffered saline. 1 M Dithiothreitol ( DTT ) 0.5 M EDTA ( pH 8.0 ) Ethidium bromide ( 10 mg / ml ) 2 × HEPES - buffered saline IPTG -20 ° C . Dissolve 3.09 g of DTT in 20 ml of 0.01 M sodium acetate ( pH 5.2 ) . Sterilize by ...
Page B-23
... EDTA Tris - phosphate ( TPE ) Tris - borate " ( TBE ) 1 × : 0.09 M Tris - phosphate 0.002 M EDTA 0.5X : 0.045 M Tris ... ( pH 8.0 ) 10x : 108 g Tris base 15.5 ml 85 % phosphoric acid ( 1.679 g / ml ) 40 ml 0.5 M EDTA ( pH 8.0 ) 5x : 54 g ...
... EDTA Tris - phosphate ( TPE ) Tris - borate " ( TBE ) 1 × : 0.09 M Tris - phosphate 0.002 M EDTA 0.5X : 0.045 M Tris ... ( pH 8.0 ) 10x : 108 g Tris base 15.5 ml 85 % phosphoric acid ( 1.679 g / ml ) 40 ml 0.5 M EDTA ( pH 8.0 ) 5x : 54 g ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml