Molecular Cloning: A Laboratory Manual, Book 3 |
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Page E-3
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PURIFICATION OF NUCLEIC ACIDS Perhaps the most basic of all procedures in molecular cloning is the purifica- tion of nucleic acids . The key step , the removal of proteins , can often be ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PURIFICATION OF NUCLEIC ACIDS Perhaps the most basic of all procedures in molecular cloning is the purifica- tion of nucleic acids . The key step , the removal of proteins , can often be ...
Page E-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. MEASUREMENT OF RADIOACTIVITY IN NUCLEIC ACIDS Radioactive isotopes are used as tracers to monitor the ... NUCLEIC ACIDS E 18 Precipitation of Nucleic Acids with Trichloroacetic Acid E 18.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. MEASUREMENT OF RADIOACTIVITY IN NUCLEIC ACIDS Radioactive isotopes are used as tracers to monitor the ... NUCLEIC ACIDS E 18 Precipitation of Nucleic Acids with Trichloroacetic Acid E 18.
Page E-30
... Nucleic acids bind to hydroxyapatite by virtue of interactions between the phosphate groups of the polynucleotide backbone and calcium residues in the resin . Bound nucleic acids can be eluted in phosphate buffers at elevated ...
... Nucleic acids bind to hydroxyapatite by virtue of interactions between the phosphate groups of the polynucleotide backbone and calcium residues in the resin . Bound nucleic acids can be eluted in phosphate buffers at elevated ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml