Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 11
Page 17-8
... ampicillin ( 100 μg / ml ) . Incubate overnight at 37 ° C ( or at 30 ° C for PEX ) . b . Inoculate 5 ml of LB medium containing ampicillin ( 100 μg / ml ) with 50 μl of each overnight culture . Incubate for 2 hours at 37 ° C ( or at 30 ...
... ampicillin ( 100 μg / ml ) . Incubate overnight at 37 ° C ( or at 30 ° C for PEX ) . b . Inoculate 5 ml of LB medium containing ampicillin ( 100 μg / ml ) with 50 μl of each overnight culture . Incubate for 2 hours at 37 ° C ( or at 30 ...
Page 17-16
... ampicillin ( 100 μg / ml ) with 50 μl of saturated culture . Incubate the culture for 2 hours at 37 ° C . b . Remove 1 ml of the uninduced culture and place in a microfuge tube . Process as described in steps c and d below . Induce the ...
... ampicillin ( 100 μg / ml ) with 50 μl of saturated culture . Incubate the culture for 2 hours at 37 ° C . b . Remove 1 ml of the uninduced culture and place in a microfuge tube . Process as described in steps c and d below . Induce the ...
Page 1-2
... Ampicillin mechanism of action , 1.6 stock solution , A.6 use as selective agent , 1.5 for maintaining expression plasmids , 17.16 Amplification , 1.3 of bacteriophage λ plaques on nitrocellu- lose filters , 2.112 of DNA by the ...
... Ampicillin mechanism of action , 1.6 stock solution , A.6 use as selective agent , 1.5 for maintaining expression plasmids , 17.16 Amplification , 1.3 of bacteriophage λ plaques on nitrocellu- lose filters , 2.112 of DNA by the ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml