Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1614
... antibiotic resistance upon both prokaryotic and eukaryotic cells . However , perhaps because the bacterial promoter contributes several upstream AUG codons , the efficiency of translation of APH mRNAs synthe- sized from these vectors is ...
... antibiotic resistance upon both prokaryotic and eukaryotic cells . However , perhaps because the bacterial promoter contributes several upstream AUG codons , the efficiency of translation of APH mRNAs synthe- sized from these vectors is ...
Page A-5
... antibiotics . Return the frozen culture to storage at -70 ° C . Incubate the plate overnight at 37 ° C . BACTERIAL ... Antibiotic Solutions Stock solutiona Working concentration stringent Bacterial Media , Antibiotics , and Bacterial ...
... antibiotics . Return the frozen culture to storage at -70 ° C . Incubate the plate overnight at 37 ° C . BACTERIAL ... Antibiotic Solutions Stock solutiona Working concentration stringent Bacterial Media , Antibiotics , and Bacterial ...
Page A-6
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ANTIBIOTICS TABLE A.1 Antibiotic Solutions Stock solutiona Working concentration stringent relaxed concentration storage plasmids plasmids Ampicillin 50 mg / ml in H2O -20 ° C 20 μg / ml ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ANTIBIOTICS TABLE A.1 Antibiotic Solutions Stock solutiona Working concentration stringent relaxed concentration storage plasmids plasmids Ampicillin 50 mg / ml in H2O -20 ° C 20 μg / ml ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml