Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1582
... cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF CDNA ...
... cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 Integrity of the mRNA 8.4 Abundant mRNAs 8.6 Low - abundance mRNAs 8.6 Methods of Enrichment 8.6 FRACTIONATION OF mRNA BY SIZE 8.8 FRACTIONATION OF CDNA ...
Page 1669
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Cloning by Expression of cDNA Clones One approach to cloning by expression is to prepare cDNA from mRNA containing the expressed gene of interest and to insert this cDNA into an ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Cloning by Expression of cDNA Clones One approach to cloning by expression is to prepare cDNA from mRNA containing the expressed gene of interest and to insert this cDNA into an ...
Page 1-6
... cDNA synthesis and cloning alternative methods mRNA - cDNA cloning , 8.27 sequential addition of different link- ers , 8.27-8.28 in single - stranded vectors , 8.32 amplification of libraries constructed in λgt10 , 8.78 constructed in ...
... cDNA synthesis and cloning alternative methods mRNA - cDNA cloning , 8.27 sequential addition of different link- ers , 8.27-8.28 in single - stranded vectors , 8.32 amplification of libraries constructed in λgt10 , 8.78 constructed in ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml