Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1584
... DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to ... Double - stranded DNA Probes 10.6 NICK TRANSLATION OF DNA 10.6 Stock Solutions Used in Nick Translation 10.7 Protocol ...
... DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to ... Double - stranded DNA Probes 10.6 NICK TRANSLATION OF DNA 10.6 Stock Solutions Used in Nick Translation 10.7 Protocol ...
Page C-1
... DNA in Solution Double - stranded DNA ( 50 μg / ml ) Molecules / ml Moles / ml Molar concentration Molar concentration of termini Bacteriophage A 9.78 × 1011 11 -12 1.62 × 10 1.62 nM 3.24 nM pBR322 1.09 × 1013 -11 1.81 x 10 18.1 nM 36.2 ...
... DNA in Solution Double - stranded DNA ( 50 μg / ml ) Molecules / ml Moles / ml Molar concentration Molar concentration of termini Bacteriophage A 9.78 × 1011 11 -12 1.62 × 10 1.62 nM 3.24 nM pBR322 1.09 × 1013 -11 1.81 x 10 18.1 nM 36.2 ...
Page E-30
... double - stranded DNA . Neoprene gasket microfuge tube circulating water heater Lucite box FIGURE E.30 Commonly Used Techniques in Molecular Cloning SEPARATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E 30.
... double - stranded DNA . Neoprene gasket microfuge tube circulating water heater Lucite box FIGURE E.30 Commonly Used Techniques in Molecular Cloning SEPARATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E 30.
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml