Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 41
Page 1572
... DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF ... Foreign DNA Fragment 1.53 FRAGMENTS CARRYING NONCOMPLEMENTARY PROTRUDING TERMINI 1.53 FRAGMENTS CARRYING IDENTICAL ...
... DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF ... Foreign DNA Fragment 1.53 FRAGMENTS CARRYING NONCOMPLEMENTARY PROTRUDING TERMINI 1.53 FRAGMENTS CARRYING IDENTICAL ...
Page 1628
... DNA that carries a drug resistance marker and transfected into the cells , or the foreign gene and drug resistance marker are " cotransfected " ( see page 16.9 ) into the cells . When cells carrying such composite structures are exposed ...
... DNA that carries a drug resistance marker and transfected into the cells , or the foreign gene and drug resistance marker are " cotransfected " ( see page 16.9 ) into the cells . When cells carrying such composite structures are exposed ...
Page 1630
... foreign DNA in large numbers of cells . Calcium phos- phate - mediated transfection is also used to establish cell lines that carry integrated copies of the foreign DNA , which are usually arranged in head - to - tail tandem arrays ...
... foreign DNA in large numbers of cells . Calcium phos- phate - mediated transfection is also used to establish cell lines that carry integrated copies of the foreign DNA , which are usually arranged in head - to - tail tandem arrays ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml