Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1579
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii 6.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Gel Electrophoresis of DNA Agarose Gel Electrophoresis 6.3 Factors ... GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xiii 6.
Page B-23
... Electrophoresis Buffers Working solution Buffer Tris - acetate ( TAE ) 1 × : 0.04 mM Tris - acetate 0.001 M EDTA ... gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all ...
... Electrophoresis Buffers Working solution Buffer Tris - acetate ( TAE ) 1 × : 0.04 mM Tris - acetate 0.001 M EDTA ... gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all ...
Page 1-16
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml