Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-17
... filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of ... NITROCELLULOSE FILTERS 18 17.
... filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of ... NITROCELLULOSE FILTERS 18 17.
Page 18-64
... nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel is placed in contact with a piece of nitrocellulose filter . The gel and its ...
... nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the older type , one side of the gel is placed in contact with a piece of nitrocellulose filter . The gel and its ...
Page 18-67
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Staining Proteins Immobilized on Nitrocellulose Filters Of the several procedures available to stain proteins immobilized on nitro- cellulose filters , only one , staining with Ponceau S ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Staining Proteins Immobilized on Nitrocellulose Filters Of the several procedures available to stain proteins immobilized on nitro- cellulose filters , only one , staining with Ponceau S ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml