Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-30
... purify the fusion protein ( see Nagai and Thøgersen 1984 ) . Store at -20 ° C until needed . a d . Activate bovine blood coagulation factor X to factor X , with Russell's viper venom ( Fujikawa et al . 1972 ) . a a e . Dissolve the purified ...
... purify the fusion protein ( see Nagai and Thøgersen 1984 ) . Store at -20 ° C until needed . a d . Activate bovine blood coagulation factor X to factor X , with Russell's viper venom ( Fujikawa et al . 1972 ) . a a e . Dissolve the purified ...
Page 18-11
... purify the IgG fraction or , in some cases , to purify the antibody of interest by binding it to its cognate antigen ... purified by another method ( e.g. , ammonium sulfate precipitation followed by chromatography on DEAE - cellulose ) ...
... purify the IgG fraction or , in some cases , to purify the antibody of interest by binding it to its cognate antigen ... purified by another method ( e.g. , ammonium sulfate precipitation followed by chromatography on DEAE - cellulose ) ...
Page 18-16
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Purification of Immunospecific Antibodies Antibodies may be purified by adsorption to , and elution from , their cognate antigen . In some cases ( e.g. , when the antigen is a protein ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Purification of Immunospecific Antibodies Antibodies may be purified by adsorption to , and elution from , their cognate antigen . In some cases ( e.g. , when the antigen is a protein ) ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml