Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1617
... DNA replication . In many cases , transient expression of cloned genes in ... vector - host system - for example , when sustained high levels of ... vector or the new gene . In the following sections , various vector - host systems that ...
... DNA replication . In many cases , transient expression of cloned genes in ... vector - host system - for example , when sustained high levels of ... vector or the new gene . In the following sections , various vector - host systems that ...
Page 1623
... DNA was cloned in prokaryotic vectors ( Howley et al . 1980 ) . The sequence of BPV - 1 DNA was then rapidly determined ( Chen et al . 1982 ) , and the genomic organization of the virus was elucidated ( Figure 16.4 ) ( for review , see ...
... DNA was cloned in prokaryotic vectors ( Howley et al . 1980 ) . The sequence of BPV - 1 DNA was then rapidly determined ( Chen et al . 1982 ) , and the genomic organization of the virus was elucidated ( Figure 16.4 ) ( for review , see ...
Page 1-8
... DNA and RNA probes , 10.64-10.65 suppression of self - ligation of cloning vectors , 1.60-1.61 treatment of cosmid vector DNA digestion with one restriction enzyme and dephosphorylation in single cos site vector , 3.28 digestion with ...
... DNA and RNA probes , 10.64-10.65 suppression of self - ligation of cloning vectors , 1.60-1.61 treatment of cosmid vector DNA digestion with one restriction enzyme and dephosphorylation in single cos site vector , 3.28 digestion with ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml