Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-55
... coli that support the growth of bacteriophage λ . All of these strains are derivatives of E. coli K12 , which codes for a restriction enzyme ( EcoK ) that cleaves DNA containing unmodified EcoK recognition sites . Although the DNA of ...
... coli that support the growth of bacteriophage λ . All of these strains are derivatives of E. coli K12 , which codes for a restriction enzyme ( EcoK ) that cleaves DNA containing unmodified EcoK recognition sites . Although the DNA of ...
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... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
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... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml