Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-16
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
Page 5-32
... restriction enzymes are supplied by the manufacturer in concen- trated form . Often , 1 μl of many enzyme preparations is sufficient to ... Enzymes Used in Molecular Cloning Many enzymes other than 5.32 Enzymes Used in Molecular Cloning.
... restriction enzymes are supplied by the manufacturer in concen- trated form . Often , 1 μl of many enzyme preparations is sufficient to ... Enzymes Used in Molecular Cloning Many enzymes other than 5.32 Enzymes Used in Molecular Cloning.
Page 5-44
... restriction enzyme and for the subsequent end - labeling . Bacteriophage T4 DNA polymerase will function at about 50 % of maximal activity in all buffers that are commonly used for digestion of DNA with restriction enzymes . However ...
... restriction enzyme and for the subsequent end - labeling . Bacteriophage T4 DNA polymerase will function at about 50 % of maximal activity in all buffers that are commonly used for digestion of DNA with restriction enzymes . However ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml