Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 88
Page 8-42
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
Page 9-56
... Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they are perfectly matched . Duplexes with a single base - pair mismatch are significantly less stable and dissociate at a lower temperature than ...
... Chapter 11 , hybrids of this length are stable enough to be detected in practice only if they are perfectly matched . Duplexes with a single base - pair mismatch are significantly less stable and dissociate at a lower temperature than ...
Page 15-72
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml