Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-58
... Cl [ pH 7.4 ] ) . Store in aliquots at -70 ° C . Packaging extracts are much cheaper to make than to buy . If you plan to construct more than one or two cDNA libraries , it would be worthwhile making a large batch of packaging extract ...
... Cl [ pH 7.4 ] ) . Store in aliquots at -70 ° C . Packaging extracts are much cheaper to make than to buy . If you plan to construct more than one or two cDNA libraries , it would be worthwhile making a large batch of packaging extract ...
Page 8-59
... pH 5.2 ) Synthetic linkers TE ( pH 7.6 and pH 8.0 ) Trichloroacetic acid ( TCA ) ( 10 % ) Tris base ( 2 M ) Tris Cl ( 2 м ; pH 7.4 ) · Tris Cl ( 1 м ; pH 7.6 ) Tris Cl ( 2 м ; pH 8.0 ) Tris Cl ( 1 M ; pH 8.3 ) X - gal Add Sephadex G ...
... pH 5.2 ) Synthetic linkers TE ( pH 7.6 and pH 8.0 ) Trichloroacetic acid ( TCA ) ( 10 % ) Tris base ( 2 M ) Tris Cl ( 2 м ; pH 7.4 ) · Tris Cl ( 1 м ; pH 7.6 ) Tris Cl ( 2 м ; pH 8.0 ) Tris Cl ( 1 M ; pH 8.3 ) X - gal Add Sephadex G ...
Page 9-20
... Cl ( pH 8.0 ) , 10 mM EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
... Cl ( pH 8.0 ) , 10 mM EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
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Common terms and phrases
agarose gel aliquots amino acid amplification bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA Biol buffer carried cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured digestion DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic DNA H₂O hybridization Incubate the reaction inserted Klenow fragment labeled ligation linkers method microfuge tube minutes at 4°C mismatched molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nucleotides oligonu oligonucleotide phagemid plaques plasmid plasmid DNA plates polymerase chain reaction pool primer probe protein purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening sequencing reactions single-stranded DNA solution specific activity Store strand of cDNA synthesis target DNA target fragment target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml